Tips and Tricks for PFE quant

[John Donovan]

Here's an interesting "trick".

Some of you may already utilize the Load Image button from the Imaging button (Acquire!) or the Digitize Image button (Digitize!) windows to load an image acquired using another software (e.g., JEOL, Cameca, Thermo, Bruker, etc. applications) into Probe for EPMA, for documentation or digitizing stage positions as described here:

https://probesoftware.com/smf/index.php?topic=42.msg9193#msg9193

The idea being that if the instrument is still at the same stage position and the same magnification (FOV) as when the loaded image was acquired, then the loaded image will be properly calibrated for the stage coordinates.

But it should be noted that one can load even images not acquired using a scanned beam, for example, an optical image from the light optics camera as seen here:



The only important thing to remember is to utilize the stage position from when the optical image was captured in the PeakSight software, and especially to set the electron optics FOV to a value that corresponds to the optical FOV.

On SX100/SXFive Cameca instruments the light optics FOV is variable, but since we use the 500 um FOV as the default optical zoom, that corresponds to about 800x magnification for the electron optics. Other optical FOVs can also be used to capture and load light optics images, just be sure to set the magnification of the instrument to the light optics FOV for proper stage calibration of the loaded images.

[Probeman]

Here's a few tips for utilizing previously acquired standard intensities from other runs.

So normally we would create a new run, then maybe load the sample setup from a previous run that had similar elements/conditions using the Acquire! window New Sample/Setup button as seen here:



This will load not only the selected sample setup, but also all run (globals) specified in that run, and optionally all the standard intensities required for that specified sample setup.

This is a great way to get going quickly with a new run and of course the imported sample setup can be further modified and/or re-standardiazed.

The other more manual method to load standard intensities requires some preparation.  Some of you may already utilize the Save Element Setup feature in Probe for EPMA to save specific element setups to the public SETUP.MDB file, so that these element setups can be recalled later by any user.

What you may not have noticed is that if the sample is a standard it will also save the standard intensity for that element. But to streamline this method, one can simply utilize the Save Sample Setups as seen here from the Analyze! window:



This button will automatically search the sample and automatically output all element setups associated with that sample including the standard (and interference and MAN) standard intensities to the (several) element setup databases.  One click is all it takes!   

Once you have saved a number of element setups, you can import them into new runs using the button shown below. Personally when using this Load Element Setup dialog, I usually just load the element setups (without the standard intensities) and then re-acquire the standards, just to be sure to get up to date standard intensities.

But if desired, one can also load individual standard intensities using the button shown below.

[John Donovan]

For this "tips and tricks" feature you will need the latest version of Probe for EPMA (as usual update from the Help | Update Probe for EPMA menu).

Sometimes a user will analyze for oxygen, but then later on would like to try simply calculating oxygen by stoichiometry.

However, if one goes into the Calculation Options dialog and clicks on the Calculate With Stoichiometric Oxygen option, they will get this warning:



To remedy this, just go to the Elements Cations dialog and first, disable the analyzed oxygen for quantification by clicking on the analyzed oxygen row, then checking the Disable Quant checkbox.

Next click on on an empty row in the Elements/Cations dialog and enter oxygen with no x-ray line (no x-ray line indicates an unanalyzed element).

Now, go back to the Calculations Options dialog and now you will be able to click the Calculate With Stoichiometric Oxygen option. After clicking the Analyze button you will now see output similar to this:

[John Donovan]

This is very much a beginner question, but I think it might help those who are just starting to use the Probe for EPMA software, because Probe for EPMA is designed completely differently than the JEOL and Cameca EPMA software.

The main point is that Probe for EPMA is intentionally designed from a "sample centric" perspective, while other EPMA software seems to be more designed from an "instrument centric" perspective. Basically when one looks at the Probe for EPMA software it appears to show a sample or samples. A sample being a collection of intensity or position data. The JEOL or Cameca software, on the other hand, appears (to me at least) to show the instrument more. Which makes sense to me because the JEOL and Cameca softwares were designed by instrument engineers, while Probe for EPMA was designed by scientists.

Basically, Probe for EPMA has two primary types of samples, each of which can contain zero to N data points. First, intensity samples (standards, unknowns and wavescans visible from the Acquire! and Analyze! windows), and second, position samples (standards, unknowns and wavescans visible from the Automate!, Digitize and Positions windows) that contain stage positions (and optionally sample conditions, setups, etc).  Here is a more detailed description:

The samples in Acquire! and Analyze! windows are intensity samples (with or without intensity data). The samples in the Automate! window are position samples (with or without position data). Once intensity samples have been created and intensity data acquired (in Acquire! manually or in Automate! automatically), these intensity samples can be viewed in the Analyze! window.

You can add position data for automated acquisition from the Automate! window using the Digitize button. When you add (stage) position data to samples in the Automate! they can then be automated and then the intensity data samples will appear in the Analyze! window for quantification.

The Acquire! window only works with the "current" or last intensity sample created (usually an unknown sample).  And the "current" sample can only be modified if it contains no intensity data (except changes for some background models, matrix corrections, software dead time, etc., etc.). So just create a new "current" sample if you want to change any acquisition conditions.

Remember, you cannot change sample conditions if the sample already contains intensity data (that would be unscientific!), but you can view the sample conditions from the Analyze! window using the Data, Elements/Cations and Conditions buttons.

[John Donovan]

Basically, Probe for EPMA has two primary types of samples, each of which can contain zero to N data points. First, intensity samples (standards, unknowns and wavescans visible from the Acquire! and Analyze! windows), and second, position samples (standards, unknowns and wavescans visible from the Automate!, Digitize and Positions windows) that contain stage positions (and optionally sample conditions, setups, etc). 

Sometimes the shortest explanation is the best explanation!

This is what one of our installers wrote to me on how they explain it:

- Acquire is where you tell it what to do.
- Automate is where you tell it where to do it.
- Analyze is where you look at what it did.

 

[Ben Buse]

My version on Owen,

-Acquire is where you create setup (with option of insitu acquistion, and record of all positions)
-Automate is where you automate
-Analyze is where you review standards and unknown results